Clinical Proteomics:Article Title: Unraveling mitochondrial pyruvate dysfunction to mitigate hyperlactatemia and lethality in sepsis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MPC1 Cell Signaling Technology Cat# 14462S; RRID: AB_2773729 Anti-MPC2 Cell Signaling Technology Cat# 46141S; RRID: AB_2799295 Anti-β-ACTIN Thermo Fisher Scientific Cat# MA5-15739; RRID: AB_10979409 Anti-PCX Cell Signaling Technology Cat# 66470S; RRID: AB_3705492 Anti-PDH Cell Signaling Technology Cat# 3205S; RRID: AB_2162926 Anti-β-TUBULIN Cell Signaling Technology Cat# 2146S; RRID: AB_2210545 Anti-PDK3 Thermo Fisher Scientific Cat# PA5-28019; RRID: AB_2545495 Anti-PDK4 Thermo Fisher Scientific Cat#PA5-13776; RRID: AB_2161497 Anti-GAPDH Cell Signaling Technology Cat# 2118T; RRID: AB_561053 Anti-P-PDHSer232 Sigma-Aldrich Cat# 1063; RRID: AB_10616070 Anti-P-PDHSer293 Cell Signaling Technology Cat# 31866S; RRID: AB_2799014 Anti-P-PDHSer300 Sigma-Aldrich Cat# ABS194; RRID: AB_11204697 Anti-GPT2 Thermo Fisher Scientific Cat# 16757-1-AP; RRID: AB_2112098 Biological samples Sepsis plasma University Hospital of Ghent N/A Chemicals, peptides, and recombinant proteins Sodium pyruvate (13C3, 99%) Cambridge Isotope Laboratories Cat# CLM-2440-0.5 Sodium pyruvate Sigma-Aldrich Cat# P2256 Ceftriaxone Sigma-Aldrich Cat# C5793 Metronidazole Sigma-Aldrich Cat# M-1547 Glutamic acid Sigma-Aldrich Cat# G8415 Malic acid Sigma-Aldrich Cat# M7397 Adenosine 5 ′ -diphosphate sodium salt Sigma-Aldrich Cat# A2754 Oligomycin Sigma-Aldrich Cat# O4876 FCCP Sigma-Aldrich Cat# C2920 Antimycin A Sigma-Aldrich Cat# A8674 Rotenone Sigma-Aldrich Cat# R8875 UK5099 Sigma-Aldrich Cat# PZ0160 Oxaloacetic acid Sigma-Aldrich Cat# O4126 Glutamic acid-13C5 Sigma-Aldrich Cat# 604860 Aminooxyacetic acid Sigma-Aldrich Cat# C13408 L-Alanine Sigma-Aldrich Cat# A7627 Sodium dichloroacetate Sigma-Aldrich Cat# 347795 Alpha lipoic acid HSN N/A Thiamine pyrophosphate Sigma-Aldrich Cat# C8754 Glucose monohydrate Sigma-Aldrich Cat# 49159 Acetyl-L-carnitine HSN N/A Critical commercial assays Pyruvate carboxylase assay kit Abbexa Cat# abx298982 PDH activity assay kit Sigma-Aldrich Cat# MAK183 Vitamin B1 (Thiamine) ELISA Kit Abbexa Cat# abx150384 Aurum total RNA mini kit Bio-Rad Cat# 732-6820 iScript cDNA synthesis kit Bio-Rad Cat# 170-01190 (Continued on next page) 20 Cell Reports 44, 116032, August 26, 2025 .. Male C57BL/6J mice (Mus musculus) were purchased from Janvier (Le Genest-St. Isle, France).
Recombinant:Article Title: Unraveling mitochondrial pyruvate dysfunction to mitigate hyperlactatemia and lethality in sepsis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MPC1 Cell Signaling Technology Cat# 14462S; RRID: AB_2773729 Anti-MPC2 Cell Signaling Technology Cat# 46141S; RRID: AB_2799295 Anti-β-ACTIN Thermo Fisher Scientific Cat# MA5-15739; RRID: AB_10979409 Anti-PCX Cell Signaling Technology Cat# 66470S; RRID: AB_3705492 Anti-PDH Cell Signaling Technology Cat# 3205S; RRID: AB_2162926 Anti-β-TUBULIN Cell Signaling Technology Cat# 2146S; RRID: AB_2210545 Anti-PDK3 Thermo Fisher Scientific Cat# PA5-28019; RRID: AB_2545495 Anti-PDK4 Thermo Fisher Scientific Cat#PA5-13776; RRID: AB_2161497 Anti-GAPDH Cell Signaling Technology Cat# 2118T; RRID: AB_561053 Anti-P-PDHSer232 Sigma-Aldrich Cat# 1063; RRID: AB_10616070 Anti-P-PDHSer293 Cell Signaling Technology Cat# 31866S; RRID: AB_2799014 Anti-P-PDHSer300 Sigma-Aldrich Cat# ABS194; RRID: AB_11204697 Anti-GPT2 Thermo Fisher Scientific Cat# 16757-1-AP; RRID: AB_2112098 Biological samples Sepsis plasma University Hospital of Ghent N/A Chemicals, peptides, and recombinant proteins Sodium pyruvate (13C3, 99%) Cambridge Isotope Laboratories Cat# CLM-2440-0.5 Sodium pyruvate Sigma-Aldrich Cat# P2256 Ceftriaxone Sigma-Aldrich Cat# C5793 Metronidazole Sigma-Aldrich Cat# M-1547 Glutamic acid Sigma-Aldrich Cat# G8415 Malic acid Sigma-Aldrich Cat# M7397 Adenosine 5 ′ -diphosphate sodium salt Sigma-Aldrich Cat# A2754 Oligomycin Sigma-Aldrich Cat# O4876 FCCP Sigma-Aldrich Cat# C2920 Antimycin A Sigma-Aldrich Cat# A8674 Rotenone Sigma-Aldrich Cat# R8875 UK5099 Sigma-Aldrich Cat# PZ0160 Oxaloacetic acid Sigma-Aldrich Cat# O4126 Glutamic acid-13C5 Sigma-Aldrich Cat# 604860 Aminooxyacetic acid Sigma-Aldrich Cat# C13408 L-Alanine Sigma-Aldrich Cat# A7627 Sodium dichloroacetate Sigma-Aldrich Cat# 347795 Alpha lipoic acid HSN N/A Thiamine pyrophosphate Sigma-Aldrich Cat# C8754 Glucose monohydrate Sigma-Aldrich Cat# 49159 Acetyl-L-carnitine HSN N/A Critical commercial assays Pyruvate carboxylase assay kit Abbexa Cat# abx298982 PDH activity assay kit Sigma-Aldrich Cat# MAK183 Vitamin B1 (Thiamine) ELISA Kit Abbexa Cat# abx150384 Aurum total RNA mini kit Bio-Rad Cat# 732-6820 iScript cDNA synthesis kit Bio-Rad Cat# 170-01190 (Continued on next page) 20 Cell Reports 44, 116032, August 26, 2025 .. Male C57BL/6J mice (Mus musculus) were purchased from Janvier (Le Genest-St. Isle, France).
Activity Assay:Article Title: Unraveling mitochondrial pyruvate dysfunction to mitigate hyperlactatemia and lethality in sepsis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MPC1 Cell Signaling Technology Cat# 14462S; RRID: AB_2773729 Anti-MPC2 Cell Signaling Technology Cat# 46141S; RRID: AB_2799295 Anti-β-ACTIN Thermo Fisher Scientific Cat# MA5-15739; RRID: AB_10979409 Anti-PCX Cell Signaling Technology Cat# 66470S; RRID: AB_3705492 Anti-PDH Cell Signaling Technology Cat# 3205S; RRID: AB_2162926 Anti-β-TUBULIN Cell Signaling Technology Cat# 2146S; RRID: AB_2210545 Anti-PDK3 Thermo Fisher Scientific Cat# PA5-28019; RRID: AB_2545495 Anti-PDK4 Thermo Fisher Scientific Cat#PA5-13776; RRID: AB_2161497 Anti-GAPDH Cell Signaling Technology Cat# 2118T; RRID: AB_561053 Anti-P-PDHSer232 Sigma-Aldrich Cat# 1063; RRID: AB_10616070 Anti-P-PDHSer293 Cell Signaling Technology Cat# 31866S; RRID: AB_2799014 Anti-P-PDHSer300 Sigma-Aldrich Cat# ABS194; RRID: AB_11204697 Anti-GPT2 Thermo Fisher Scientific Cat# 16757-1-AP; RRID: AB_2112098 Biological samples Sepsis plasma University Hospital of Ghent N/A Chemicals, peptides, and recombinant proteins Sodium pyruvate (13C3, 99%) Cambridge Isotope Laboratories Cat# CLM-2440-0.5 Sodium pyruvate Sigma-Aldrich Cat# P2256 Ceftriaxone Sigma-Aldrich Cat# C5793 Metronidazole Sigma-Aldrich Cat# M-1547 Glutamic acid Sigma-Aldrich Cat# G8415 Malic acid Sigma-Aldrich Cat# M7397 Adenosine 5 ′ -diphosphate sodium salt Sigma-Aldrich Cat# A2754 Oligomycin Sigma-Aldrich Cat# O4876 FCCP Sigma-Aldrich Cat# C2920 Antimycin A Sigma-Aldrich Cat# A8674 Rotenone Sigma-Aldrich Cat# R8875 UK5099 Sigma-Aldrich Cat# PZ0160 Oxaloacetic acid Sigma-Aldrich Cat# O4126 Glutamic acid-13C5 Sigma-Aldrich Cat# 604860 Aminooxyacetic acid Sigma-Aldrich Cat# C13408 L-Alanine Sigma-Aldrich Cat# A7627 Sodium dichloroacetate Sigma-Aldrich Cat# 347795 Alpha lipoic acid HSN N/A Thiamine pyrophosphate Sigma-Aldrich Cat# C8754 Glucose monohydrate Sigma-Aldrich Cat# 49159 Acetyl-L-carnitine HSN N/A Critical commercial assays Pyruvate carboxylase assay kit Abbexa Cat# abx298982 PDH activity assay kit Sigma-Aldrich Cat# MAK183 Vitamin B1 (Thiamine) ELISA Kit Abbexa Cat# abx150384 Aurum total RNA mini kit Bio-Rad Cat# 732-6820 iScript cDNA synthesis kit Bio-Rad Cat# 170-01190 (Continued on next page) 20 Cell Reports 44, 116032, August 26, 2025 .. Male C57BL/6J mice (Mus musculus) were purchased from Janvier (Le Genest-St. Isle, France).
Enzyme-linked Immunosorbent Assay:Article Title: Unraveling mitochondrial pyruvate dysfunction to mitigate hyperlactatemia and lethality in sepsis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MPC1 Cell Signaling Technology Cat# 14462S; RRID: AB_2773729 Anti-MPC2 Cell Signaling Technology Cat# 46141S; RRID: AB_2799295 Anti-β-ACTIN Thermo Fisher Scientific Cat# MA5-15739; RRID: AB_10979409 Anti-PCX Cell Signaling Technology Cat# 66470S; RRID: AB_3705492 Anti-PDH Cell Signaling Technology Cat# 3205S; RRID: AB_2162926 Anti-β-TUBULIN Cell Signaling Technology Cat# 2146S; RRID: AB_2210545 Anti-PDK3 Thermo Fisher Scientific Cat# PA5-28019; RRID: AB_2545495 Anti-PDK4 Thermo Fisher Scientific Cat#PA5-13776; RRID: AB_2161497 Anti-GAPDH Cell Signaling Technology Cat# 2118T; RRID: AB_561053 Anti-P-PDHSer232 Sigma-Aldrich Cat# 1063; RRID: AB_10616070 Anti-P-PDHSer293 Cell Signaling Technology Cat# 31866S; RRID: AB_2799014 Anti-P-PDHSer300 Sigma-Aldrich Cat# ABS194; RRID: AB_11204697 Anti-GPT2 Thermo Fisher Scientific Cat# 16757-1-AP; RRID: AB_2112098 Biological samples Sepsis plasma University Hospital of Ghent N/A Chemicals, peptides, and recombinant proteins Sodium pyruvate (13C3, 99%) Cambridge Isotope Laboratories Cat# CLM-2440-0.5 Sodium pyruvate Sigma-Aldrich Cat# P2256 Ceftriaxone Sigma-Aldrich Cat# C5793 Metronidazole Sigma-Aldrich Cat# M-1547 Glutamic acid Sigma-Aldrich Cat# G8415 Malic acid Sigma-Aldrich Cat# M7397 Adenosine 5 ′ -diphosphate sodium salt Sigma-Aldrich Cat# A2754 Oligomycin Sigma-Aldrich Cat# O4876 FCCP Sigma-Aldrich Cat# C2920 Antimycin A Sigma-Aldrich Cat# A8674 Rotenone Sigma-Aldrich Cat# R8875 UK5099 Sigma-Aldrich Cat# PZ0160 Oxaloacetic acid Sigma-Aldrich Cat# O4126 Glutamic acid-13C5 Sigma-Aldrich Cat# 604860 Aminooxyacetic acid Sigma-Aldrich Cat# C13408 L-Alanine Sigma-Aldrich Cat# A7627 Sodium dichloroacetate Sigma-Aldrich Cat# 347795 Alpha lipoic acid HSN N/A Thiamine pyrophosphate Sigma-Aldrich Cat# C8754 Glucose monohydrate Sigma-Aldrich Cat# 49159 Acetyl-L-carnitine HSN N/A Critical commercial assays Pyruvate carboxylase assay kit Abbexa Cat# abx298982 PDH activity assay kit Sigma-Aldrich Cat# MAK183 Vitamin B1 (Thiamine) ELISA Kit Abbexa Cat# abx150384 Aurum total RNA mini kit Bio-Rad Cat# 732-6820 iScript cDNA synthesis kit Bio-Rad Cat# 170-01190 (Continued on next page) 20 Cell Reports 44, 116032, August 26, 2025 .. Male C57BL/6J mice (Mus musculus) were purchased from Janvier (Le Genest-St. Isle, France).
cDNA Synthesis:Article Title: Unraveling mitochondrial pyruvate dysfunction to mitigate hyperlactatemia and lethality in sepsis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MPC1 Cell Signaling Technology Cat# 14462S; RRID: AB_2773729 Anti-MPC2 Cell Signaling Technology Cat# 46141S; RRID: AB_2799295 Anti-β-ACTIN Thermo Fisher Scientific Cat# MA5-15739; RRID: AB_10979409 Anti-PCX Cell Signaling Technology Cat# 66470S; RRID: AB_3705492 Anti-PDH Cell Signaling Technology Cat# 3205S; RRID: AB_2162926 Anti-β-TUBULIN Cell Signaling Technology Cat# 2146S; RRID: AB_2210545 Anti-PDK3 Thermo Fisher Scientific Cat# PA5-28019; RRID: AB_2545495 Anti-PDK4 Thermo Fisher Scientific Cat#PA5-13776; RRID: AB_2161497 Anti-GAPDH Cell Signaling Technology Cat# 2118T; RRID: AB_561053 Anti-P-PDHSer232 Sigma-Aldrich Cat# 1063; RRID: AB_10616070 Anti-P-PDHSer293 Cell Signaling Technology Cat# 31866S; RRID: AB_2799014 Anti-P-PDHSer300 Sigma-Aldrich Cat# ABS194; RRID: AB_11204697 Anti-GPT2 Thermo Fisher Scientific Cat# 16757-1-AP; RRID: AB_2112098 Biological samples Sepsis plasma University Hospital of Ghent N/A Chemicals, peptides, and recombinant proteins Sodium pyruvate (13C3, 99%) Cambridge Isotope Laboratories Cat# CLM-2440-0.5 Sodium pyruvate Sigma-Aldrich Cat# P2256 Ceftriaxone Sigma-Aldrich Cat# C5793 Metronidazole Sigma-Aldrich Cat# M-1547 Glutamic acid Sigma-Aldrich Cat# G8415 Malic acid Sigma-Aldrich Cat# M7397 Adenosine 5 ′ -diphosphate sodium salt Sigma-Aldrich Cat# A2754 Oligomycin Sigma-Aldrich Cat# O4876 FCCP Sigma-Aldrich Cat# C2920 Antimycin A Sigma-Aldrich Cat# A8674 Rotenone Sigma-Aldrich Cat# R8875 UK5099 Sigma-Aldrich Cat# PZ0160 Oxaloacetic acid Sigma-Aldrich Cat# O4126 Glutamic acid-13C5 Sigma-Aldrich Cat# 604860 Aminooxyacetic acid Sigma-Aldrich Cat# C13408 L-Alanine Sigma-Aldrich Cat# A7627 Sodium dichloroacetate Sigma-Aldrich Cat# 347795 Alpha lipoic acid HSN N/A Thiamine pyrophosphate Sigma-Aldrich Cat# C8754 Glucose monohydrate Sigma-Aldrich Cat# 49159 Acetyl-L-carnitine HSN N/A Critical commercial assays Pyruvate carboxylase assay kit Abbexa Cat# abx298982 PDH activity assay kit Sigma-Aldrich Cat# MAK183 Vitamin B1 (Thiamine) ELISA Kit Abbexa Cat# abx150384 Aurum total RNA mini kit Bio-Rad Cat# 732-6820 iScript cDNA synthesis kit Bio-Rad Cat# 170-01190 (Continued on next page) 20 Cell Reports 44, 116032, August 26, 2025 .. Male C57BL/6J mice (Mus musculus) were purchased from Janvier (Le Genest-St. Isle, France).
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